Interactively draw and construct gate objects

cyto_gate_draw allows to users to interactive draw gates around populations which are returned as flowCore gate objects. The flowFrame and flowSet methods simply return the constructed gates as a list of flowCore-compatible gate objects, whilst the GatingSet method automatically applies the constructed gates to the GatingSet and saves the constructed gates in an openCyto gatingTemplate for future use. See cyto_gate_edit, cyto_gate_remove and cyto_gate_rename to manipulate constructed gates and modify their entries in the gatingTemplate.

# S3 method for GatingSet
cyto_gate_draw(
  x,
  parent = "root",
  alias = NULL,
  channels = NULL,
  type = NULL,
  gatingTemplate = NULL,
  overlay = NA,
  group_by = "all",
  select = NULL,
  negate = FALSE,
  display = 25000,
  axis = "x",
  label = TRUE,
  plot = TRUE,
  popup = TRUE,
  axes_limits = "machine",
  gate_point_shape = 16,
  gate_point_size = 1,
  gate_point_col = "red",
  gate_point_col_alpha = 1,
  gate_line_type = 1,
  gate_line_width = 2.5,
  gate_line_col = "red",
  gate_line_col_alpha = 1,
  ...
)

# S3 method for flowSet
cyto_gate_draw(
  x,
  alias = NULL,
  channels = NULL,
  type = NULL,
  overlay = NA,
  group_by = "all",
  select = NULL,
  negate = FALSE,
  display = 25000,
  axis = "x",
  label = TRUE,
  plot = TRUE,
  popup = TRUE,
  axes_limits = "machine",
  gate_point_shape = 16,
  gate_point_size = 1,
  gate_point_col = "red",
  gate_point_col_alpha = 1,
  gate_line_type = 1,
  gate_line_width = 2.5,
  gate_line_col = "red",
  gate_line_col_alpha = 1,
  ...
)

# S3 method for flowFrame
cyto_gate_draw(
  x,
  alias = NULL,
  channels = NULL,
  type = NULL,
  overlay = NA,
  negate = FALSE,
  display = 25000,
  axis = "x",
  label = TRUE,
  plot = TRUE,
  popup = TRUE,
  axes_limits = "machine",
  gate_point_shape = 16,
  gate_point_size = 1,
  gate_point_col = "red",
  gate_point_col_alpha = 1,
  gate_line_type = 1,
  gate_line_width = 2.5,
  gate_line_col = "red",
  gate_line_col_alpha = 1,
  ...
)

Arguments

x	object of class `flowFrame`, `flowSet` or `GatingSet`.
parent	name of the `parent` population to extract for gating when a `GatingSet` object is supplied.
alias	the name(s) of the populations to be gated. If multiple population names are supplied (e.g. `c("CD3,"CD4)`) multiple gates will be returned. `alias` is `NULL` by default which will halt the gating routine.
channels	vector of channel names to use for plotting, can be of length 1 for 1-D density histogram or length 2 for 2-D scatter plot.
type	vector of gate type names used to construct the gates. Multiple gate types are supported but should be accompanied with an `alias` argument of the same length (i.e. one `type` per `alias`). Supported gate types are `polygon, rectangle, ellipse, threshold, boundary, interval, quadrant and web` which can be abbreviated as upper or lower case first letters as well. Default `type` is `"interval"` for 1D gates and `"polygon"` for 2D gates.
gatingTemplate	name of `gatingTemplate` csv file to which the `gatingTemplate` entries for the `GatingSet` method should be saved.
overlay	name(s) of the populations to overlay or a `flowFrame`, `flowSet`, `list of flowFrames` or `list of flowSets` containing populations to be overlaid onto the plot(s).
group_by	vector of `cyto_details` column names (e.g. c("Treatment","Concentration") indicating how the samples should be grouped prior to gating, set to the length of x by default to construct a single gate for all samples. If group_by is supplied a different gate will be constructed for each group. This argument only applies to `flowSet` or `GatingSet` objects.
select	designates which samples will be plotted and used for determining the best location to set the drawn gate(s). Filtering steps should be comma separated and wrapped in a list. Refer to `cyto_select`.
negate	logical indicating whether a gatingTemplate entry should be made for the negated population (i.e. all events outside the constructed gates), set to FALSE by default. If negate is set to TRUE, a name for the negated population MUST be supplied at the end of the alias argument.
display	fraction or number of events to display in the plot during the gating process, set to 25 000 events by default.
axis	indicates whether the `"x"` or `"y"` axis should be gated for 2-D interval gates.
label	logical indicating whether to include `cyto_plot_label` for the gated population(s), `TRUE` by default.
plot	logical indicating whether a plot should be drawn, set to `TRUE` by default.
popup	logical indicating whether the plot should be constructed in a pop-up window, set to TRUE by default.
axes_limits	options include `"auto"`, `"data"` or `"machine"` to use optimised, data or machine limits respectively. Set to `"machine"` by default to use entire axes ranges. Fine control over axes limits can be obtained by altering the `xlim` and `ylim` arguments.
gate_point_shape	shape to use for selected gate points, set to `16` by default to use filled circles. See `pch` for alternatives.
gate_point_size	numeric to control the size of the selected gate points, set to 1 by default.
gate_point_col	colour to use for the selected gate points, set to "red" by default.
gate_point_col_alpha	numeric [0,1] to control the transparency of the selected gate points, set to 1 by default to use solid colours.
gate_line_type	integer [0,6] to control the line type of gates, set to `1` to draw solid lines by default. See `lty` for alternatives.
gate_line_width	numeric to control the line width(s) of gates, set to `2.5` by default.
gate_line_col	colour to use for gates, set to `"red"` by default.
gate_line_col_alpha	numeric [0,1] to control the transparency of the selected gate lines, set to 1 by default to use solid colours.
...	additional arguments for `cyto_plot`.

Value

flowFrame and flowSet methods return a list of flowCore gate objects per group in group_by, whilst the GatingSet applies the constructed gates directly to the GatingSet and adds appropriate entries into the specified gatingTemplate. The GatingSet method does not return the constructed gates but instead invisibly returns the gatingTemplate entries.

Author

Dillon Hammill, Dillon.Hammill@anu.edu.au

Examples

if (FALSE) {
# Gate drawing requires an interactive R session
library(CytoExploreRData)

# Load in samples
fs <- Activation
gs <- GatingSet(fs)

# Apply compensation
gs <- compensate(gs, fs[[1]]@description$SPILL)

# Transform fluorescent channels
trans <- estimateLogicle(gs[[4]], cyto_fluor_channels(fs))
gs <- transform(gs, trans)

# Gate using cyto_gate_draw
gt_gating(Activation_gatingTemplate, gs)

# draw gates using cyto_gate_draw
cyto_gate_draw(gs,
  parent = "Dendritic Cells",
  channels = c("Alexa Fluor 488-A", "Alexa Fluor 700-A"),
  alias = c("CD8+ DC", "CD4+ DC"),
  gatingTemplate = "Example-gatingTemplate.csv",
  type = "rectangle",
  contour_lines = 15
)

# Constructed gate applied directly to GatingSet
cyto_nodes(gs)
}

Arguments

Value

See also

Author

Examples