Convert between cytometry objects
cyto_convert(x, ...) # S3 method for flowFrame cyto_convert(x, return = "flowFrame", ...) # S3 method for flowSet cyto_convert(x, return = "flowSet", ...) # S3 method for GatingHierarchy cyto_convert(x, parent = "root", return = "GatingHierarchy", ...) # S3 method for GatingSet cyto_convert(x, parent = "root", return = "GatingSet", ...)
| x | |
|---|---|
| ... | not in use. |
| return | either 'flowFrame', 'flowSet', 'GatingHierarchy', 'GatingSet', coerced 'flowFrame list' or coerced 'flowSet list'. GatingSet and flowSet objects can also be converted to a 'list of flowFrames'. |
| parent | name of parent population to extract from
|
object specified by 'return' argument.
Dillon Hammill, Dillon.Hammill@anu.edu.au
# Load in CytoExploreRData to access data library(CytoExploreRData) # Convert flowSet to 'list of flowFrames' cyto_convert(Activation, "list of flowFrames")#> $Activation_1.fcs #> flowFrame object 'Activation_1.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_2.fcs #> flowFrame object 'Activation_2.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_3.fcs #> flowFrame object 'Activation_3.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_4.fcs #> flowFrame object 'Activation_4.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0.00 262144 #> $P2 FSC-H NA 262144 0.00 262144 #> $P3 FSC-W NA 262144 0.00 262144 #> $P4 SSC-A NA 262144 -35.64 262144 #> $P5 SSC-H NA 262144 0.00 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_5.fcs #> flowFrame object 'Activation_5.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 -111 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 -111 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_6.fcs #> flowFrame object 'Activation_6.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_7.fcs #> flowFrame object 'Activation_7.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_8.fcs #> flowFrame object 'Activation_8.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_9.fcs #> flowFrame object 'Activation_9.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_10.fcs #> flowFrame object 'Activation_10.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_11.fcs #> flowFrame object 'Activation_11.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_12.fcs #> flowFrame object 'Activation_12.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_13.fcs #> flowFrame object 'Activation_13.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_14.fcs #> flowFrame object 'Activation_14.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_15.fcs #> flowFrame object 'Activation_15.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_16.fcs #> flowFrame object 'Activation_16.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_17.fcs #> flowFrame object 'Activation_17.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_18.fcs #> flowFrame object 'Activation_18.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_19.fcs #> flowFrame object 'Activation_19.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_20.fcs #> flowFrame object 'Activation_20.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_21.fcs #> flowFrame object 'Activation_21.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 -111 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_22.fcs #> flowFrame object 'Activation_22.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_23.fcs #> flowFrame object 'Activation_23.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_24.fcs #> flowFrame object 'Activation_24.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_25.fcs #> flowFrame object 'Activation_25.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_26.fcs #> flowFrame object 'Activation_26.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_27.fcs #> flowFrame object 'Activation_27.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 -111 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_28.fcs #> flowFrame object 'Activation_28.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 -111 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_29.fcs #> flowFrame object 'Activation_29.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_30.fcs #> flowFrame object 'Activation_30.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 -111 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_31.fcs #> flowFrame object 'Activation_31.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_32.fcs #> flowFrame object 'Activation_32.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 261 keywords are stored in the 'description' slot #> #> $Activation_33.fcs #> flowFrame object 'Activation_33.fcs' #> with 50000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 -111 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 -111 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111.00 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111.00 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -84.48 262144 #> $P17 APC-Cy7-A CD11c 262144 -111.00 262144 #> $P18 Time NA 262144 0.00 262144 #> 261 keywords are stored in the 'description' slot #># Convert flowSet to 'flowFrame' cyto_convert(Activation, "flowFrame")#> flowFrame object 'anonymous' #> with 1650000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262144 0 262144 #> $P2 FSC-H NA 262144 0 262144 #> $P3 FSC-W NA 262144 0 262144 #> $P4 SSC-A NA 262144 0 262144 #> $P5 SSC-H NA 262144 0 262144 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262144 -111 262144 #> $P15 Alexa Fluor 647-A CD44 262144 -111 262144 #> $P16 Alexa Fluor 700-A CD4 262144 -111 262144 #> $P17 APC-Cy7-A CD11c 262144 -111 262144 #> $P18 Time NA 262144 0 262144 #> 2 keywords are stored in the 'description' slot# Convert GatingSet to flowFrame cyto_convert(GatingSet(Activation), "flowFrame", parent = "root")#> flowFrame object 'anonymous' #> with 1650000 cells and 18 observables: #> name desc range minRange maxRange #> $P1 FSC-A NA 262143 0 262143 #> $P2 FSC-H NA 262143 0 262143 #> $P3 FSC-W NA 262143 0 262143 #> $P4 SSC-A NA 262143 0 262143 #> $P5 SSC-H NA 262143 0 262143 #> ... ... ... ... ... ... #> $P14 Qdot 605-A NA 262143 -111 262143 #> $P15 Alexa Fluor 647-A CD44 262143 -111 262143 #> $P16 Alexa Fluor 700-A CD4 262143 -111 262143 #> $P17 APC-Cy7-A CD11c 262143 -111 262143 #> $P18 Time NA 262143 0 262143 #> 2 keywords are stored in the 'description' slot